Figure 7.

Caspase inhibitor Z-VAD-fmk reverses dimethylarsenic acid-induced caspase cascade in MA-10 cells. MA-10 cells were pretreated with Z-VAD-fmk for 2 h and co-treated with or without 1 mM dimethylarsenic acid for 24 h. Cleaved caspase-8, −9 and −3 protein expression was detected by western blotting. (A) Data are representative of one experiment that was further repeated three times. The integrated optical densities of cleaved (B) caspase-8, (C) −9 and (D) −3 protein expression were analyzed after normalization to β-actin. Data in (B), (C) and (D) represent the mean ± standard error of the mean of three separate experiments. *P<0.05.