Table II.
Statistical analysis of mRNA expression of genes in diffuse/poorly cohesive gastric cancers relative to the peri-tumoral tissues.
| A, Growth factors and receptors (n=10) | |||
|---|---|---|---|
| Genes | Nontumoral gastric tissues(n=11)b | Diffuse/poorly cohesive carcinoma (n=13)b | P-valuea |
| IGF1 | 1 (0.42–2.93) | 7.37 (0.97–12.75) | 0.00037 |
| IGF2 | 1 (046–3.13) | 2.26 (0.80–19.16) | 0.024 |
| IGF1R | 1 (0.55–1.46) | 1.10 (0.63–1.55) | 0.79 (NS) |
| IGF2R | 1 (0.79–1.33) | 1.43 (0.85–1.65) | 0.0071 |
| IRS1 | 1 (0.59–1.90) | 1.50 (0.91–2.71) | 0.0084 |
| IRS2 | 1 (0.62–1.69) | 1.13 (0.52–4.44) | 0.51 (NS) |
| FGF7 | 1 (0.25–2.62) | 2.16 (1.10–3.45) | 0.0019 |
| FGFR1 | 1 (0.55–2.95) | 1.94 (0.96–3.53) | 0.060 (NS) |
| ERRB2 | 1 (0.43–1.41) | 1.62 (0.87–2.74) | 0.0016 |
| ERRB3 | 1 (0.19–1.70) | 0.91 (0.51–2.55) | 0.98 (NS) |
| B, EMT and migration (n=10) | |||
| Genes | Nontumoral gastric tissues(n=11)b | Diffuse/poorly cohesive carcinoma (n=13)b | P-valuea |
| VIM | 1 (0.65–1.64) | 1.62 (0.88–2.37) | 0.0013 |
| CDH1 | 1 (0.05–1.22) | 0.78 (0.01–1.06) | 0.04 |
| SNAI1 | 1 (0.29–2.07) | 2.39 (0.90–4.92) | 0.0019 |
| SLUG/SNAI2 | 1 (0.61–2.0) | 3.02 (1.15–4.06) | 0.0013 |
| TWIST2 | 1 (0.58–2.82) | 2.30 (0.62–3.29) | 0.046 |
| TGFB1 | 1 (0.47–1.23) | 2.05 (1.47–4.27) | 0.000034 |
| RUNX3 | 1 (0.00–2.23) | 1.77 (0.00–4.91) | 0.0065 |
| ZEB2 | 1 (0.58–1.41) | 1.70 (0.84–2.97) | 0.00046 |
| CXCR4 | 1 (0.47–3.56) | 3.14 (1.52–7.30) | 0.00086 |
| CXCL12 | 1 (0.26–3.49) | 1.05 (0.30–3.19) | 0.75 (NS) |
| C, Cell proliferation and migration (n=7) | |||
| Genes | Nontumoral gastric tissues(n=11)b | Diffuse/poorly cohesive carcinoma (n=13)b | P-valuea |
| MMP2 | 1 (0.68–1.99) | 3.21 (1.47–6.15) | 0.000057 |
| MMP9 | 1 (029–2.76) | 2.01 (0.92–4.13) | 0.015 |
| SPP1 osteopontin | 1 (0.43–2.04) | 4.12 (1.75–89.35 | 0.000057 |
| CD44 | 1 (0.57–1.89) | 1.73 (1.02–2.81) | 0.0019 |
| RHOB | 1 (0.30–2.84) | 0.52 (0.32–1.23) | 0.21 (NS) |
| RHOA | 1 (0.05–5.29) | 8.65 (0.03–20.39) | 0.0026 |
| MKI67 | 1 (0.1–3.71) | 3.76 (1.34–10.83) | 0.00078 |
| D, Angiogenesis (n=6) | |||
| Genes | Nontumoral gastric tissues(n=11)b | Diffuse/poorly cohesive carcinoma (n=13)b | P-valuea |
| VEGFA 165 | 1 (0.68–1.56) | 0.86 (0.58–2.09) | 0.71 (NS) |
| VEGFA 189 | 1 (0.49–1.63) | 0.67 (0.42–1.00) | 0.0091 |
| FLT1 | 1 (0.64–2.20) | 1.08 (0.62–2.07) | 0.91 (NS) |
| KDR | 1 (0.63–2.63) | 1.11 (0.67–1.47) | 0.40 (NS) |
| VEGFC | 1 (0.44–1.57) | 1.38 (0.86–2.60) | 0.0071 |
| NRP1 | 1 (0.57–1.87) | 1.94 (1.21–3.27) | 0.00037 |
a
Mann Whitney's U test.
b
Median (range) of gene mRNA expression levels. NS, not significant; EMT, epithelial-mesenchymal transition.