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. 2019 May 21;18(1):637–644. doi: 10.3892/ol.2019.10391

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Copyright: © Yang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Effects of SPANXA1 on BCAP cell proliferation, migration, invasion and apoptosis. (A) Microscopy images of cells transfected with 50 nmol/l siRNA and control cells (magnification, ×400). (B) After transfection, SPANXA1 expression was decreased. (C) Proliferation of BCPAP cells treated with iodine at 1.0×10⁻⁶ mol/l as assessed by an MTT assay; SPANXA1-knockdown BCPAP cells exhibited suppressed proliferation. Migration of BCPAP cells with downregulated SPANXA1 was evaluated using (D) a Transwell assay and (E) invasion was analysed using a scratch-wound assay. Downregulation of SPANXA1 contributed to the inhibition of migration and invasion of iodine-treated BCPAP cells. Magnification, ×20. (F) Cell apoptosis results were obtained by flow cytometry and suggested that downregulation of SPANXA1 increased apoptosis in iodine-treated BCPAP cells (1.0×10⁻⁶ mol/l). *P<0.05 and **P<0.01 vs. the control group. NC, negative control; SPANXA1, sperm protein associated with the nucleus, X-linked, family member A1; si, small interfering; OD, optical density.