Figure 4.

Effects of the PI3K/AKT signalling pathway. (A) Downregulation of SPANXA1 suppresses PI3K and p-AKT expression in iodine-treated BCPAP cells (1.0×10⁻⁶ mol/l). (B) PI3K and p-AKT protein expression were increased in BCPAP cells incubated with iodine at 1.0×10⁻⁶ mol/l. Applying PI3K inhibitor LY294002 to iodine-treated cells suppressed biological behaviours, including cell proliferation, invasion and migration, but promoted apoptosis, as demonstrated by (C) MTT, (D) wound healing and (E) Transwell assays, as well as (F) flow cytometry, respectively. Magnification, ×20. *P<0.05 and **P<0.01 vs. the control group. SPANXA1, sperm protein associated with the nucleus, X-linked, family member A1; p, phosphorylated; PI3K, phosphoinositide 3-kinase; t, total.