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. 2019 May 28;10:687. doi: 10.3389/fpls.2019.00687

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Copyright © 2019 Carbonell.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Secondary siRNA-based silencing tools in plants. (A). The atasiRNA/syn-tasiRNA pathway. TAS1 precursor sequence is in black, with trigger miR173 target site (TS) in light gray. AtasiRNA/syn-tasiRNA sequences targeting RNA 1 are shown in dark and light blue; atasiRNA/syn-tasiRNA sequences targeting RNA 2 are shown in dark and light green; miR173 sequence is shown in yellow. Promoter and terminator sequences are shown with a dark gray arrow and box, respectively. Participating proteins are represented with colored ovals. (B) The MIGS pathway. Sequences corresponding to gene fragments 1 and 2 are in diverse blue and green tonalities, respectively. Other details are as in (A).