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. 2019 May 22;10:635. doi: 10.3389/fpls.2019.00635

Figure 2.

Figure 2

FLS2-StRem1.3 BiFC complexes appear to associate with ER-PM contact sites in N. benthamiana leaf cortical cells. (A) FLS2-StRem1.3 BiFC complexes co-expressed with endoplasmic reticulum maker SP-tagRFP-HDEL. (B) Dynamic motion of the ER network labeled by ER SP-tagRFP-HDEL and stable localization of puncta of FLS2-StRem1.3 BiFC complexes revealed by kymograph analysis. (C) Kymograph analysis of FLS2-StRem1.3 BiFC complexes co-expressed with SP-tagRFP-HDEL. In (B,C), kymographs were created from a short time-lapse series (80 s) across transect lines ~30 μm in length (shown in white). (D) FLS2-StRem1.3 BiFC complexes co-expressed with Arabidopsis ER-PM tethering protein Synaptogamin 1 (SYT1) fused to tagRFP. All scale bars (white in A,D; black in B,C) represent 10 μm.