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. 2019 May 22;10:635. doi: 10.3389/fpls.2019.00635

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Copyright © 2019 Tao, Waletich, Arredondo and Tyler.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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A C-terminal peripheral PM-binding domain is required for ER-PM tethering by Arabidopsis SYT1 in N. benthamiana leaf cortical cells. (A) Explanatory schematic of reconstitution of SYT1 ER-PM tethering using BiFC complexes. (B,C) Distribution and kymograph analysis of full length SYT1 or SYT1 reconstituted using Venus BiFC complexes. (D,E) Distribution and kymograph analysis of SYT1 lacking the C-terminal peripheral PM-binding domain C2AC2B labeled by either full-length YFP or a free Venus BiFC complex. (F,G) Distribution and kymograph analysis of SYT1n fused to WT-type StRem1.3 or PM-non-binding mutant StRem1.3^* via Venus BiFC complexes. All kymographs were created as described in Figure 2. All scale bars represent 10 μm.