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. 2019 May 22;10:635. doi: 10.3389/fpls.2019.00635

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Copyright © 2019 Tao, Waletich, Arredondo and Tyler.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Model for the production of ER-PM tethering complexes via BiFC. Normally, newly synthesized RLK protein FLS2 is targeted to the PM through the ER and transported to the PM via the coat protein complex II (COPII) system. Co-expression of FLS2 and StRem1.3 in BiFC constructs results in rapid spontaneous formation of Venus BiFC complexes tethered to the PM. PM tethering blocks ER-anchored FLS2 from delivery to the PM, resulting in artificial PM-ER complexes. Artificial ER-PM tethering could be created by pairing any ER-transiting integral membrane protein (IMP) with any peripheral membrane protein (PMP) in a BiFC complex.