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(A) SDS-PAGE and (B) Western blot (anti-his) analysis of the purified Pfs230C1 protein for the three culture media at 72 h harvest. SFM4 (Lanes 1–2), ESF921 (Lanes 3–4), and Ex-cell 420 (lanes 5–6) were evaluated under non-reducing (Lanes 1, 3, 5) and reducing (lanes 2, 4, 6) conditions. (M) denotes marker lane. Arrows indicate the target Pfs230C1 protein.
