Fig. 5.

SDS-PAGE and Western blot analysis of final purified Pfs230C1. (A) SDS-PAGE under non-reducing and reducing conditions followed by silver stain. (B) Western blot with anti-his antibody. (A–B) Lanes 1–2 representative Pfs230C1 from the original Super Sf9 process [16], and Lanes 3–4 Pfs230C1 derived from 20 L optimized process. Non-reducing (Lanes 1, 4) and reducing (Lanes 2, 3) conditions. Western blot analysis of (C) Pfs230C1 and (D) native parasite extracts with mouse monoclonal antibody 15A4-1B12 under reducing (R) and non-reducing (NR) conditions. Different molecular weight marker standards are utilized for panels C and D given the difference in molecular weight of target protein. Expected molecular weight of native Pfs230 is ∼363 kDa, however, since protease inhibitors are not utilized, multiple bands are visible.