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(A) Size exclusion HPLC analysis (B) Ion exchanged HPLC analysis and (C) Reverse Phase HPLC analysis of three 20 L consistency lots preparation of Pfs230C1. Blue trace indicates Lot #1, red trace indicates Lot #2 and green trace indicates Lot #3 of final purified Pfs230C1. In SE-HPLC analysis of Pfs230C1 (Panel A), second peak is absorption peak of buffer components (Data not shown).
