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SDS-PAGE Freeze/thaw stability analysis of final purified Pfs230C1 under non-reducing (A) and reducing (B) conditions followed by silver stain. Reference Pfs230C1 from previous [16] Super Sf9 process (Lanes 1–2). Pfs230C1 from 20 L optimized process after one (Lanes 3–4) three (Lanes 5–6) and five (lanes 7–8) freeze/thaw cycles. Proteins loaded at 1 μg (Lanes 1, 3, 5, 7) and 5 μg (Lanes 2, 4, 6, 8).
