Table 1.
Name | Manufacturer | Catalog number | Host | IHC/IF |
---|---|---|---|---|
EZH2∗ | Cell Signaling (Danvers, MA) | 5246 | Rabbit | 1:400 |
FOXA2 | Abcam (Cambridge, MA) | Ab108422 | Rabbit | 1:250 |
Ki-67 | Abcam | Ab16667 | Rabbit | 1:200 |
VIM | Cell Signaling | 5741 | Rabbit | 1:200 |
ACTA2 | Cell Signaling | 19245 | Rabbit | 1:500 |
KRT8 | DSHB (Iowa City, IA) | TROMA-I | Rat | 1:200 |
KRT14 | Thermo Fisher Scientific (Waltham, MA) | PA5-16722 | Rabbit | 1:400 |
KRT14 | Santa Cruz Biotechnology (Dallas, TX) | SC-53253 | Mouse | 1:100 |
KRT5 | BioLegend (San Diego, CA) | 905501 | Rabbit | 1:3000 |
KRT6A | BioLegend | 905701 | Rabbit | 1:1000 |
ΔNp63 | BioLegend | 619001 | Rabbit | 1:500 |
ACTA2, α-smooth muscle actin; DSHB, Developmental Studies Hybridoma Bank; EZH2, enhancer of zeste 2 polycomb repressive complex 2 subunit; FOXA2, forkhead box A2; IF, immunofluorescence; IHC, immunohistochemistry; KRT, cytokeratin; ΔNp63, tumor protein 63 not containing an N-terminal transactivation domain; VIM, vimentin.
Western blot analysis: 1:1000