Supplemental Figure S2.

A: Renal HEK cells [untreated control (Con; top panels)] or treated with linoleic acid (LA; 100 μmol/L; bottom panels) in serum-free media for 5 hours and stained with NucView 488 caspase-3 (Nuc), propidium iodide (PI), and Hoechst 33342. B: Quantification of the PI/Hoechst 33342 fluorescence (red lines) and NucView/Hoechst 33342 (green lines) shows a significant increase in caspase-3 activity and PI uptake with LA at 4 and 6 hours. Data are expressed as means ± SEM (B). n = 4 independents experiments (B). ^∗P < 0.05 versus control on analysis of variance.