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. 2019 Jun;189(6):1226–1240. doi: 10.1016/j.ajpath.2019.02.015

Figure 2.

Figure 2

Comparison of the lipolysis by pancreatic lipases using conditions relevant to human pancreatitis. A–C: Parameters [bile acid (A), nonesterified fatty acids (NEFAs; B), and linoleic acid (C)] measured in human pancreatic fluid collections of biliary and nonbiliary origin. D and E: Comparison of the ability of 1 μg/mL of human (D) and murine (E) pancreatic lipases to hydrolyze 600 μmol/L glyceryl trilinoleate (GTL) at pH 7.4 in the absence of bile acids. F and G: Effect of different concentrations of sodium taurocholate on human (F) and mouse (G) carboxyl ester lipase (CEL), as measured by the free fatty acid (FFA) generated by GTL (600 μmol/L) lipolysis over 1 hour. H–J: Comparison of the rate of FFA production with glycerol generation by GTL (600 μmol/L) lipolysis among the three pancreatic lipases. Although there is an initial delay in generating glycerol by pancreatic triacylglycerol lipase (PNLIP), by 4 hours, both glycerol and FFA are equivalent. Data are expressed as means ± SEM. CON, control; PNLIPRP2, pancreatic lipase-related protein 2.