Figure 5.

Loss of Trr COMPASS-like Complex Activity Induces Abnormal ISC Accumulation

(A–D) Wild-type (A), trr RNAi (B), lpt RNAi (C), or Utx RNAi (D) at 9 days AHS clones.

(E and F) Average number of cells (E) and ISCs (F) per clone from (A)–(D).

(G and G′) Utx was ubiquitously expressed but enriched in esg>GFP+ cells and in EE (esg>GFP− diploid cells).

(H) Mean Utx staining fluorescence intensity normalized by the mean DAPI staining in ISCs, EEs, and ECs (polyploid cells) from (G) and (G′).

(I and I′) Lpt was ubiquitously expressed but enriched in EE cells (diploid; esg>GFP−).

(J and J′) Trr was uniformly expressed in all midgut cell types.

In (E), (F), and (H), a two-tailed Mann-Whitney statistical test was used. Mean values in red; error bars, SEM; n = non-significant, *p < 0.05, ***p < 0.001, ****p < 0.0001. Scale bars, 20 μm.