JZL195 elicits CA1 LTD via glutamate receptors. Plots of normalized
fEPSP slopes in anesthetized rats (a, b) or mice (d) show that
JZL195 injection at 0 min elicits CA1 LTD in animals, which is
blocked by pretreatment with ceftriaxone (a), Ro25-6981, ifenprodil
(b) or Tat-GluR2 (d). Representative fEPSP traces before (1) and
after (2) treatment are shown above each plot. (c) Graphs and
immunoblotting (bottom photos) show a decrease in GluR2 at the
synaptic surface of CA1 neurons after JZL195 injection. (e) Bar
charts summarizing the average percent change of fEPSP slope before
(1) and after (2) vehicle or JZL195 injection as depicted in panels
a, b and d. All summary graphs show means ± SEMs;
n = numbers of animals assessed in each group (a)
to (d). *p < 0.01 vs. vehicle (a) and (b) or
Tat-GluR2S (d), Bonferronni post-hoc test after
one-way ANOVA ((b): F2,8 = 41.090,
p < 0.01) or t test (a),
(c) and (d).