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Immunocytochemical results at Day 21 of culture for bovine AF cells seeded on smooth PCL surfaces. Scaffolds were treated with primary antibodies targeting (a) ACAN, (b) COL 1, (c) COL 2, (d) TNMD. Alexa Fluor® 647 was used for secondary antibody, and cells were counterstained with ethidium bromide. Cell nuclei are shown in cyan to enhance contrast. Scale bars=100 μm
