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. 2019 Feb 27;47(10):e55. doi: 10.1093/nar/gkz137

Figure 5.

Figure 5.

Antibody-chromatin delivery with plasmid DNA encoding a CRISPR/Cas9 system. (A) Agarose gel electrophoresis of DPH1 gRNA/Cas9 plasmid DNA (lanes 1–3) and chromatin (lanes 4–6) after partial DNA hydrolysis by MNase with increasing incubation time (20s, 80s and 270s). (B) Representative microscopic images of DT resistant MCF7 cell clones after treatment with vehicle (PBS control), targeted control plasmid chromatin (LeY-Chromatin (eGFP)), non-targeted Cas9/DPH1 gRNA plasmid chromatin (CD33-Chromatin (Cas9/DPH1 gRNA)), targeted Cas9/DPH1 gRNA plasmid chromatin (LeY-Chromatin (Cas9/DPH1 gRNA)); DT resistant colonies were only observed after treatment with targeted DPH1 gRNA/Cas9 plasmid chromatin.