Table 1.

The binding affinity between ExoG and various nucleic acid substrates as measured by fluorescence polarization

ExoG	Substrate	Kd (μM)a	Kd fold-changeb
H140A	R2-DNA/DNA	1.03 ± 0.086	5.42
	DNA/DNA	1.32 ± 0.079	6.95
	RNA/DNA	0.19 ± 0.010	1.00
H140A/N176A	R2-DNA/DNA	0.93 ± 0.063	7.75
	DNA/DNA	0.95 ± 0.063	7.92
	RNA/DNA	0.12 ± 0.008	1.00
H140A/F168A	R2-DNA/DNA	3.15 ± 0.259	12.60
	DNA/DNA	3.93 ± 0.188	15.72
	RNA/DNA	0.25 ± 0.014	1.00

^aThe best-fit K_d values acquired from three replicates of the experiment as shown in Supplementary Figure S6.

^bFold-change in K_d value compared to RNA/DNA substrate for each ExoG mutant.