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. 2019 Mar 27;47(10):5049–5060. doi: 10.1093/nar/gkz207

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 3. — Changes in Rluc expression observed as the VEGF PQS with no base modifications replaced key sequence elements in the SV40 promoter. (A) Promoter diagram to identify the color key for the plots. Relative response ratios (RRR = Rluc/luc) measured when the VEGF PQS replaced key elements of the SV40 promoter in plasmids that were transfected in (B) U87, (C) HepG2 or (D) HeLa cell lines. The values were normalized to the native SV40 promoter for comparison (normalized RRR = RRR_expt/RRR_SV40). The luciferase expression levels were determined using a dual-glo luciferase assay conducted 48 h post transfection. The RRRs measured were normalized to the native psiCHECK2 plasmid RRR in the same cell line to account for cell-specific differences in expression.