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. 2019 Mar 27;47(10):5126–5140. doi: 10.1093/nar/gkz189

Figure 3.

Figure 3.

In the presence of ATPγS, wild-type RecA nucleoprotein filaments retain the 5′-proximal end preference for successful strand exchange, and exhibit no end preference for unsuccessful reactions. (A and B) This set of experiments used 5′-labeled 229 nt single-stranded DNA and 229/149 nt surface-bound hybrid DNA. (A) Histogram for initial BM values for successful strand exchange in the case of wild-type RecA filaments with beads attached at the 5′-end of the bound DNA in the presence of non-hydrolyzable ATPγS (N = 68). The experimental data are consistent with the 5′-proximal end preference (red line). (B) Histogram detailing initial BM values for the unsuccessful strand exchange with wild-type RecA in ATPγS (N = 229), suggesting no end preference.