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. 2019 Mar 27;47(10):5126–5140. doi: 10.1093/nar/gkz189

Figure 4.

Figure 4.

RecAΔC17 nucleoprotein filaments have no end preference for successful strand exchange in the presence of ATP, but retain the 5′-end preference in the presence of ATPγS. (A–D) This two sets of experiments used 5′-labeled 229 nt single-stranded DNA and 229/149 nt surface-bound hybrid DNA. (A) Histogram detailing initial BM values for successful RecAΔC17 nucleoprotein filaments with beads attached to the 5′-end of the bound DNA in the presence of ATP (N = 84). The experimental data indicate no end preference (black line). (B) Histogram detailing initial BM values for the unsuccessful RecAΔC17 nucleoprotein filaments (N = 182) in the presence of ATP shows no end preference. (C) Histogram detailing initial BM values for successful strand exchange by RecAΔC17 nucleoprotein filaments in the presence of ATPγS (N = 80) is most consistent with the 5′-proximal end preference model (red line). (D) Histogram detailing initial BM values for unsuccessful strand exchange by RecAΔC17 nucleoprotein filaments in the presence of ATPγS (N = 202) is most consistent with the no end preference model (black line).