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. 2019 Apr 2;47(10):5325–5340. doi: 10.1093/nar/gkz199

Figure 1.

Figure 1.

Mesenchymal stem cells (MSCs) possess a unique circular RNA (circRNA) expression profile compared to differentiated fibroblasts. (A) Principal component analysis of the circRNA expression profiles of bone marrow MSCs (BM-MSCs), cord blood MSCs (CB-MSCs) and human skin fibroblasts (HSFs). Each cell type was assayed in triplicate. (B) Heat map of the most differentially expressed circRNAs (cut-off was set to -1.5 > fold change >1.5) between MSCs and HSFs, with columns representing circRNAs and rows representing samples. The circRNAs were classified according to the Pearson correlation (Z-score). (C) A volcano plot was used to visualize differential circRNA expression between MSCs and HSFs. The red dots represent the differentially expressed circRNAs with statistical significance (P < 0.01); downward arrows indicate downregulated circRNAs and upward arrows indicate upregulated circRNAs. (D) The relative transcriptional level of the top 10 differentially expressed circRNAs was confirmed by qRT-PCR analysis of RNAs extracted from independent biological replicates of BM-MSCs, CB-MSCs and HSFs. Data in panel (D) are shown as means ± standard deviation (n = 3), statistical analysis was performed by one-way analysis of variance followed by the Dunnett correction; *P < 0.05, **P < 0.01, ***P < 0.001.