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. 2019 Mar 20;47(10):5001–5015. doi: 10.1093/nar/gkz191

Figure 5.

Figure 5.

TZF1 acts downstream of PRR proteins to modulate TOR signaling. (A) The primary root length reduction of TZF1 OE prr579 was not additive of prr579 and TZF1 OE upon activation of TOR signaling by sugars. Three-day-old seedlings grown in liquid sugar-free 1/2 MS were treated with 15 mM Glc or Suc for 3 days in weak light (22°C, LD). (Scale bar: 0.5 cm). (B) Quantitative analysis of the primary root length in (A). Red arrows indicate the hypocotyl and root junctions. Data represent mean ± s.e.m. of 16 plants. The result was shown from one of three independent experiments with similar results. Root lengths were measured by using ImageJ. (C) DIC imaging of root meristem zones. Scale bar: 50 μm. The blue arrow indicates the root quiescent cells, and the red arrow indicate the transition between meristem zone and elongation zone. (D) Quantitative analysis of the root meristem size in (C). Data represent mean ± s.e.m. of 15 plants. The result was shown from one of three independent experiments with similar results. The root meristem size was measured by using ImageJ. Different letters in (B) and (D) represent the significant difference as P < 0.05 by one-way ANOVA with SPSS software. Uppercase letters compare with each other in 15 mM Glc treatment condition, and lowercase letters compare with each other in 15 mM Suc treatment condition.