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. 2019 Mar 20;47(10):5001–5015. doi: 10.1093/nar/gkz191

Figure 7.

Figure 7.

Diminished TOR-signaling affects root architecture in TZF1 OE line and prr579 mutant. (A) Reduced lateral root number in prr579 mutant and TZF1 OE line. The lower panel is a close-up view of the upper panel. The seedlings were grown on solid MS plates with 1% sucrose at 22°C under LD condition for 14 days. (B) Quantitative analysis of lateral root number per plant as shown in (A). Data represent mean ± s.e.m. of 15 plants. The result was shown from one of three independent experiments with similar results. The asterisk indicates significant difference as ** P < 0.01 by t-test. (C) Primary root elongation was reduced in both prr579 mutant and TZF1 OE line. The seedlings were grown on MS plates for 14 days in weak light (22°C, LD). Scale bar: 1 cm. Red arrows indicate the hypocotyl and root junctions. (D) Quantitative analysis of primary root length. The root lengths were measured by using ImageJ. Mock, sugar-free; Man, mannitol; Glc, glucose; Suc, sucrose. Data represent mean ± s.e.m. of 15 plants. The result was shown from one of three independent experiments with similar results. Different letters represent significant difference as P < 0.05 by one-way ANOVA with SPSS software. Uppercase letters compare with each other in 15 mM Glc treatment condition, and lowercase letters compare with each other in 15 mM Suc treatment condition.