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. 2019 Jun 3;10:160. doi: 10.1186/s13287-019-1263-4

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Characterization of cell surface markers and morphology of BM-MSCs. A The morphology of ALL MSCs (1a–1d) and AA MSCs (2a–2d). The morphology of the primary passage (P0) and the P4 passage MSCs of ALL (upper panel) and AA (lower panel) at different time points (primary culture—a: day 7, b: day 10, c: day 14; passage culture—d: day 7 P4 MSCs) (× 10). B-1, B-2 Cell surface markers of P4 MSCs were analyzed using FCM. Antibodies against CD90, CD29, CD105, and CD166 (upper panel), and CD34, CD45, CD19, and HLA-DR (lower panel) were used to characterize ALL MSCs (B-1) and AA MSCs (B-2). The green curves indicated the isotype controls. The purple curves indicated the expression levels of MSC surface markers