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. 2019 Jun 3;10:160. doi: 10.1186/s13287-019-1263-4

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Effect of OCT4 overexpression on the expression of stemness-related transcription factors (TFs) by FCM and RT-PCR. A Comparison of the expression levels of stemness-related TFs (NANOG, SOX2, OCT4) by FCM between the OCT4 overexpression and the parental untransfected MSCs. B The expression levels of stemness-related TFs detected by FCM at different subcloning time points in OCT4 overexpression and untransfected MSCs. The results represented mean ± SD. C mRNA of the three TFs were analyzed by RT-PCR to verify the FCM data. GAPDH serves as an internal control. RT(-) refers to the template replaced by water. Lanes 2–4 refer to the three different ALL samples with OCT4 stable overexpression. D The morphology changes of ALL MSCs after transfection with OCT4 (b) and after the third time subcloning (c), and (a) refers to the untransfected P4 MSCs (× 10)