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. 2019 Jun 3;23:198. doi: 10.1186/s13054-019-2493-7

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — M2 microglia/macrophage polarization in rat brain 7 days after reperfusion. Rrepresentative images of Arg1 (M2 marker; orange), Iba1 (microglia marker; green), and DAPI (blue) multiple staining at the inner boundary of the infarction 7 days after reperfusion (a); quantification of M2 phenotype (Arg1⁺ Iba1⁺) (b) and microglia/macrophages (Iba1⁺) (c) at the inner boundary of the infarction. Significance was indicated with ****p < 0.0001. Treatment of argon significantly promoted the M2 microglia/macrophage polarization (p = 0.000095) in rat CNS 7 days after reperfusion. Results were represented as mean ± SD, n = 8 for tMCAO Ar group and n = 7 for tMCAO N₂ group