Fig. 5.

Exogenous methylated nucleosides are incorporated into mammalian DNA. a Exogenously supplied 6mA nucleosides were incorporated by C2C12 cells as assessed by UHPLC-ms/ms. Cells were continuously supplemented in their media with 1 mM A or 6mA nucleoside for 5 days. ND is not detected at our limit of detection < 0.00003%. This graph represents the mean +/− SEM for three biological replicates where undigested signal was subtracted from digested signal. b Gel-purified C2C12 gDNA reveals incorporation of exogenous 6mA when continuously supplemented in the media (1 mM for 5 days). Graph represents the mean +/− SEM for two biological replicates where undigested signal was subtracted from digested signal