Table 3.
Cell death modes of MCF-7 cells induced by postbiotic UL4
Untreated control (%) | 15% (v/v) (%) | 30% (v/v) (%) | |
---|---|---|---|
24 h | |||
Viable cells | 86.7a ± 1.5 | 84.8a ± 1.3 | 52.2b ± 6.3 |
Apoptotic cells | 11.9a ± 1.4 | 13.9ab ± 2.0 | 30.1ab ± 4.2 |
Necrotic cells | 1.4a ± 1.1 | 1.4a ± 0.7 | 17.7bc ± 2.2 |
48 h | |||
Viable cells | 92.2a ± 1.2 | 79.1a ± 0.6 | 24.7d ± 7.2 |
Apoptotic cells | 6.5a ± 1.5 | 17.3a ± 0.5 | 65.9c ± 9.8 |
Necrotic cells | 1.3a ± 0.7 | 3.7ab ± 0.1 | 9.4ac ± 2.6 |
72 h | |||
Viable cells | 88.1a ± 1.1 | 48.6c ± 0.1 | 14.7d ± 3.5 |
Apoptotic cells | 9.6a ± 0.6 | 38.8b ± 1.2 | 60.0c ± 4.3 |
Necrotic cells | 2.3a ± 0.5 | 12.7abc ± 1.3 | 25.3c ± 7.7 |
Notes: Percentages of viable, early apoptotic, and late apoptotic and necrotic cells were scored based on their morphological features. The data represent the mean of percentage of cells. Values shown represent mean ± SEM. Values within the same row and column of a particular cell type sharing a common superscript letter are not significantly different (P > 0.05). The cell death mode of MCF-7 cells was analyzed by fluorescent microscopy