Fig. 3.

Overexpression of HMGB1 eliminates the effects of miR-449a on cell proliferation, migration, and IL-6 production in RA-FLS. RA-FLS were transfected with miR-NC or cotransfected with miR-449a and the HMGB1 expression vector (HMGB1) or HMGB1 negative control vector (HMGB1-NC) for the indicated times. The expression of HMGB1 was detected using qPCR (a) and western blotting (b). Cell proliferation was detected via a CCK-8 assay (c). Cell migration was performed in transwells. Scale bar: 50 μm. (d, e). IL-6 production was detected by ELISA (f, n = 5). Data expressed as the mean ± SD, β-actin was used as the endogenous control for the mRNA qPCR and western blotting. One-way ANOVA was used for data analysis. *p < 0.05, **p < 0.01, ***p < 0.001