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. 2019 Jun 3;19:232. doi: 10.1186/s12870-019-1790-2

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 10 — Phenotype and molecular analysis of ib-miR156 over-expressing transgenic Arabidopsis. a, b Phenotype of wild type Arabidopsis (WT) and ib-miR156-OE transgenic Arabidopsis plant in pots. c, d Phenotype of WT and ib-miR156-OE transgenic Arabidopsis plant under stereomicroscope (the magnification is 0.35 times). e the relative expression levels of ib-miR156, SPL9, CHS, CHI, DFR and ANS were measured by qRT-PCR. The expression level of each gene in WT was set as 1.0. Relative expression was calculated using the 2^-ΔΔCt method. The bars represented the mean ± SD values of three biological experiments. * and ** indicated a statistically significant difference between WT and ib-miR156-OE transgenic Arabidopsis at P < 0.05 and 0.01, respectively