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. 2019 Jun 3;20:446. doi: 10.1186/s12864-019-5826-7

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Read mapping. a Average proportion of reads mapping to miRNAs (red), other RNA species (blue) and discarded for being too long, short or adapter dimer (grey) in each RNA extraction/library preparation combination (n = 6). b Total reads were downsampled to 5 million and the number of reads mapping to miRNAs was determined for each RNA extraction and library preparation method (n = 6). Error bars show standard error of the mean, significance was determined using Friedman test and Dunn’s multiple comparison test. * p < 0.05 ***p < 0.001, where no p-value shown there was no significance. c Percentages of non-miRNA reads mapping to specific RNA types