Fig. 4.

Effect of RNA extraction on miRNA diversity. a Number of individual miRNAs detected from an equal number of reads (5 million). miRNAs with a minimum of 2 RPM were counted, error bars show standard error of the mean (n = 6), significance determined by applying Friedman test and Dunn’s multiple comparison test. * p < 0.05 ***p < 0.001 where no p-value shown there was no significance. b Number of different miRNAs detected (minimum 10 reads) with increasing read depth up to 5,000,000 reads