Table 2.
miRNAs differentially detected between NEXTflex and QIAseq library preparations. Significantly differentially detected miRNAs were identified by a two-group paired comparison (n = 6) between NEXTflex and QIAseq library preparations on both MagnaZol and miRNeasy RNA extractions, with Baggerley’s test and Bonferroni correction. miRNAs consistently differing between library preparations from MagnaZol and miRNeasy are listed, with positive fold change indicating higher detection in QIAseq and negative fold-change higher detection in NEXTflex
| miRNA | MagnaZol Fold Change | MagnaZol p-value | miRNeasy Fold Change | miRNeasy p-value | Higher In |
|---|---|---|---|---|---|
| let-7d-3p | −9.6 | 8.88E-40 | −3.8 | 1.64E-11 | NF |
| let-7 g-5p | −3.1 | 2.82E-13 | −3.0 | 8.66E-25 | NF |
| let-7i-3p | 18.1 | 4.67E-08 | 5.7 | 1.85E-03 | Q |
| mir-103a-1-3p | 2.1 | 6.69E-05 | 2.5 | 6.86E-04 | Q |
| mir-103a-2-3p | 2.1 | 8.77E-05 | 2.6 | 5.71E-04 | Q |
| mir-10a-5p | −4.7 | 3.38E-11 | −2.5 | 7.99E-08 | NF |
| mir-10b-5p | −5.9 | 1.14E-23 | −4.0 | 4.24E-07 | NF |
| mir-1260b-5p | −20.8 | 1.46E-08 | −8.5 | 2.85E-03 | NF |
| mir-126-5p | 9.6 | 5.66E-09 | 16.9 | 0.00 | Q |
| mir-130a-3p | 4.9 | 3.27E-08 | 4.5 | 4.74E-03 | Q |
| mir-130b-5p | −11.1 | 1.42E-03 | −6.3 | 6.83E-10 | NF |
| mir-142-3p | 71.7 | 0.00 | 40.2 | 3.53E-06 | Q |
| mir-142-5p | 11.5 | 8.96E-04 | 4.6 | 8.39E-06 | Q |
| mir-148b-3p | 2.7 | 5.36E-06 | 2.7 | 5.46E-08 | Q |
| mir-151a-5p | −11.4 | 2.99E-07 | −16.1 | 1.24E-07 | NF |
| mir-152-3p | 6.4 | 9.85E-06 | 4.8 | 1.79E-02 | Q |
| mir-15a-5p | 2.8 | 5.38E-03 | 3.1 | 3.59E-02 | Q |
| mir-16-1-5p | 15.1 | 0.00 | 13.2 | 0.00 | Q |
| mir-16-2-3p | − 57.3 | 9.57E-28 | −39.8 | 2.97E-52 | NF |
| mir-16-2-5p | 15.1 | 0.00 | 13.1 | 0.00 | Q |
| mir-17-3p | 66.7 | 2.89E-05 | 6.0 | 8.12E-03 | Q |
| mir-185-3p | 5.5 | 5.65E-03 | 4.1 | 1.93E-12 | Q |
| mir-192-5p | 3.0 | 1.52E-04 | 2.7 | 8.94E-12 | Q |
| mir-194-1-5p | 9.7 | 2.94E-10 | 7.3 | 5.31E-09 | Q |
| mir-194-2-5p | 8.7 | 2.60E-10 | 4.9 | 7.26E-08 | Q |
| mir-196b-5p | 3.9 | 6.59E-12 | 10.9 | 4.83E-13 | Q |
| mir-19a-3p | 13.1 | 1.56E-08 | 3.2 | 4.26E-03 | Q |
| mir-19b-1-3p | 19.9 | 2.87E-08 | 6.7 | 5.58E-05 | Q |
| mir-19b-2-3p | 19.3 | 1.98E-08 | 6.4 | 3.44E-05 | Q |
| mir-20a-5p | 20.2 | 3.89E-09 | 11.7 | 1.32E-07 | Q |
| mir-20b-5p | 19.5 | 1.78E-10 | 19.8 | 0.00 | Q |
| mir-223-3p | 5.7 | 0.00 | 4.4 | 5.61E-08 | Q |
| mir-26b-5p | 2.1 | 2.54E-04 | 2.5 | 0.00 | Q |
| mir-28-5p | −17.0 | 5.26E-09 | −13.9 | 1.78E-06 | NF |
| mir-29b-1-3p | 15.0 | 2.20E-13 | 5.2 | 1.24E-05 | Q |
| mir-29b-2-3p | 14.7 | 6.59E-13 | 5.8 | 9.28E-07 | Q |
| mir-324-5p | 4.5 | 1.44E-06 | 4.7 | 6.02E-05 | Q |
| mir-340-3p | −13.7 | 1.48E-06 | −30.2 | 2.19E-04 | NF |
| mir-361-5p | 4.0 | 5.11E-08 | 3.9 | 3.48E-08 | Q |
| mir-451a-5p | −6.5 | 5.56E-68 | −4.8 | 4.41E-12 | NF |
| mir-486-1-3p | −9.2 | 8.16E-20 | −5.8 | 3.23E-26 | NF |
| mir-486-2-3p | −8.7 | 3.04E-18 | −6.4 | 2.40E-35 | NF |
| mir-495-3p | −13.5 | 6.11E-09 | −44.3 | 1.29E-06 | NF |
| mir-502-3p | −6.1 | 8.01E-03 | −2.4 | 4.58E-05 | NF |
| mir-543-3p | −32.5 | 1.14E-02 | −14.6 | 2.84E-04 | NF |
| mir-652-3p | −15.0 | 8.76E-45 | −17.8 | 1.97E-05 | NF |
| mir-885-5p | −20.5 | 4.21E-02 | −9.2 | 2.29E-03 | NF |
| mir-93-5p | 4.5 | 6.26E-06 | 4.3 | 0.00 | Q |
| mir-98-5p | −4.6 | 8.03E-05 | −4.0 | 1.54E-10 | NF |