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. 2019 Jun 3;12:81. doi: 10.1186/s12920-019-0527-2

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Determination of UGT1A1*28 (TA)₇ promoter repeat by next-generation sequencing (NGS). a Schematics of manual assessment of aligned reads within the UGT1A1 promoter region, and multimodal frequency distribution pattern of UGT1A1*28 genotype. b Confirmation of NGS determined UGT1A1*28 genotype by TaqMan assay