Fig. 3.

Daily microbial community analysis over the 4-day incubation period within generation 20. The analysis was performed on the three communities that showed highest chitinase activity by the end of the 4 days and which would have been used to inoculate the next generation. a Simpson's index of diversity of the 16S (left) and 18S rRNA gene (right) amplicon analysis. The scale ranges between 0.38 (low) and 0.93 (high). b Community relative abundance over the 4-day incubation period. Only ASVs with abundance above 1% in at least one time point are shown. The abundance for each ASV is a mean value from the three communities. ASVs were classified to genus level using the SILVA database (v132). Names in brackets were not identifiable with the standard analysis pipeline and were identified through a BLAST search of the NCBI database. c Five 16S and 18S rRNA gene ASVs that contributed the most to the community variations over time according to a SIMPER analysis. The percentage of variation to which each ASV contributes is indicated. Error bars represent the standard deviations of the three communities used to inoculate the next generation