Fig. 1.

Effects of T. gondii infection on ATP-induced apoptosis of human SF268 neural cells. SF268 cells were infected with the RH, ME49 or VEG strain of T. gondii (MOI = 3) for 2 or 22 h, followed by ATP induction for 4 or 6 h. The cells were harvested at 6 or 28 h post-infection for apoptosis measurement by flow cytometry after annexin V-FITC/PI staining. Representative flow cytometry data are presented in panel a and quantified in panels b1 and b2. The experiments were repeated four times. The values were analyzed using the Kruskal–Wallis H-test and Bonferroni correction (*P < 0.05, **P < 0.01)