Fig. 4.

Inhibition of ATP-induced apoptosis of human SF268 neural cells by the T. gondii virulence factor ROP18. SF268 cells were infected with RH or RH-Δrop18 tachyzoites (MOI = 13) and followed by ATP induction at 12 h post-infection. The controls included SF268 cells without any treatment (N) and SF268 cells with ATP induction but without T. gondii infection (ATP). Apoptosis was measured by flow cytometry after annexin V-FITC/PI staining. Representative flow cytometry data are presented in panel a and quantified in panel b. The experiments were repeated four times. The values were analyzed using the Kruskal–Wallis H-test and Bonferroni correction (*P < 0.05, **P < 0.01)