Fig. 9.

TgROP18 inhibition of Cyt C release from mitochondria to the cytosol in ATP-treated SF268 cells. SF268 cells were harvested and lysed, and the mitochondria and cytosol were fractionated. a, b Western blot analysis of protein distributions in the mitochondria and cytosol. The data represent one of the four repeated experiments. c Densitometric analysis of the western blotting images. In four repeated experiments, the ratios of Cyt C/actin, Cyt C/COXIV, Bcl2/COXIV, Bax/COXIV and Bcl2/Bax were calculated independently. Statistical analysis was performed using the Kruskal–Wallis H-test and Bonferroni correction (**P < 0.01, *P < 0.05) (c1, cytosolic fraction; c2–c5 mitochondrial fraction)