Figure 1.

Cell viability and expression of AMPK and related molecules in parent and PEM‐resistant cells. (A) Paired cells, NCI‐H28/H28‐PEM, NCI‐H226/H226‐PEM, MSTO‐211H/211H‐PEM, and NCI‐H2452/H2452‐PEM cells, were treated with various concentrations of PEM as indicated for 72 h and cell viability was measured with the WST assay. Average and standard error bars are shown (n = 3). IC₅₀ values with SE are shown as pg·mL⁻¹ and asterisks indicate statistical significance of PEM sensitivity between parent and PEN‐resistant cells (P < 0.05, ANOVA). (B) Paired parent and PEM‐resistant cells were examined for expression levels of AMPK and the related molecules with Western blot analysis as indicated. Tubulin‐α was used as a loading control.