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. 2019 Apr 10;4(4):6551–6559. doi: 10.1021/acsomega.9b00327

Figure 7.

Figure 7

Three-component phase-separating proteins on the phase-separated lipid membrane. (a) PRM × 4, GRB2-GST, and LAT sequence peptide were added from solution to interact with ternary mixture GUVs with phase separation properties (green and gray for separated domains). LAT sequence peptide (green) was labeled with Atto488 at the C-terminal and had a polyhistidine tag at N-terminal, so it spontaneously anchored itself to the membranes by strongly binding to Ni-NTA lipid. (b–g) Matching typical images of the Atto488 protein channel and the TexasRed-DHPE lipid channel of GUVs with multiple-phase domains. Scale bars are 5 μm. Lipid compositions were 42% DOPC, 29% DPPC, 14% cholesterol, 5% DOPS, 10% Ni-DGS, 0.1% TR-DHPE.