Fig. 6.

Dose-dependent uptake and metabolism of β-apo-8′-carotenal in Caco-2 cells. Differentiated Caco-2 cells were incubated with serum-free medium containing 1, 2, 3, 4, or 5 μM of β-apo-8′-carotenal at 37°C for 4 h. After incubation, the extracts from cells were analyzed using HPLC-DAD. The results shown represent an individual experiment and the points are the mean ± standard deviation of three or more wells. Furthermore, the data shown above have been confirmed with three independent experiments.