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HPLC-DAD analysis of cell extract after incubation with β-apo-10′-carotenal. A: Representative HPLC-DAD chromatogram of the uptake of 1 μM of β-apo-10′-carotenal into fully differentiated Caco-2 cells after incubation at 37°C for 4 h. B: UV-vis spectra of β-apo-10′-carotenal and its cellular metabolites, the major one of which was identified as 5,6-epoxy-β-apo-10′-carotenol (compound A) and the other is likely a dihydro-β-apo-10′-carotenol (compound B).
