Fig. 9.

A: Time course of uptake and metabolism of β-apo-10′-carotenal in Caco-2 cells. B: Effect of incubation time on the disappearance of β-apo-10′-carotenal from medium. Differentiated Caco-2 cells were incubated with serum-free medium containing 1 μM of β-apo-10′-carotenal at 37°C for 15, 30, 45, and 60 min. After incubation, the extracts from medium and cells were analyzed by HPLC-DAD. The results shown represent an individual experiment and the points are the mean ± standard deviation of three or more wells. Furthermore, the data shown in A have been confirmed with two independent experiments.