Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jun 3;10:2421. doi: 10.1038/s41467-019-10432-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — DHX36 is mainly cytoplasmic and does not directly interact with ribosomes. a Quantification of transgenic FLAG/HA(FH)-DHX36 expression upon 16 h induction with tetracycline (Tet.). TUBA4A-normalized DHX36 quantities are indicated below. b Endogenous (Endo.), as well as transgenic FH-tagged DHX36 isoform 1 (Iso. 1) and 2 (Iso. 2) show mainly cytoplasmic localization in biochemical fractionation experiments from HEK293 cells. Cytoplasmic (C) and nuclear (N) fractions were probed with anti-HISTH2B (nuclear marker), anti-TUBA4A (cytoplasmic marker), and anti-CANX (endoplasmic reticulum marker) antibodies. Endogenous DHX36 and transgenic FH-DHX36 isoforms 1 and 2 were detected with anti-DHX36 antibody and anti-HA antibody, respectively. c DHX36 can be co-purified with polyadenylated RNA. The RBP FMR1 served as a positive, TUBA4A as a negative control, respectively. d UV absorbances at 254 nm of RNase A-treated (red) and untreated (blue) HEK293 cell extracts separated by sucrose gradient centrifugation are shown. Peaks of UV absorbance corresponding to 40S, 60S, 80S ribosomes, and polysomes are indicated. Western blots probed for DHX36, RPL22, and EIF4A1 are shown below. Source data are provided as a Source Data file