Table 4.

Primers and probes used for the detection and qualification by vitality qPCR.

STRAIN		Primer/Probe (5′-3′)	Fragment length
P. gingivalis	Forward	GCG CTC AAC GTT CAG CC	68 bp
	Reverse	CAC GAA TTC CGC CTG C
	Probe	CAC TGA ACT CAA GCC CGG CAG TTT CAA
P. intermedia	Forward	CGG TCT GTT AAG CGT GTT GTG	99 bp
	Reverse	CAC CAT GAA TTC CGC ATA CG
	Probe	TGG CGG ACT TGA GTG CAC GC
F. nucleatum	Forward	GGA TTT ATT GGG CGT AAA GC	162 bp
	Reverse	GGC ATT CCT ACA AAT ATC TAC GAA
	Probe	CTC TAC ACT TGT AGT TCC G
A. actinomycetemcomitans	Forward	GAA CCT TAC CTA CTC TTG ACA TCC GAA	80 bp
	Reverse	TGC AGC ACC TGT CTC AAA GC
	Probe	AGA ACT CAG AGA TGG GTT TGT GCC TTA GGG