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. 2019 Jun 4;9:8275. doi: 10.1038/s41598-019-44722-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Overexpression of Znf703 mRNA inhibits neural crest migration. Embryos were injected unilaterally at 2- or 4-cell stage with either 0.5 ng Znf703 (A–F’) or control mCherry. (A–C) Znf703 mRNA reduces the lateral and anterior expression of Sox10, Twist, and Foxd3 in stage 19 embryos (shown in anterior view). Injected side is to the right of the dotted line, and is indicated by the magenta β-gal lineage tracer. (D’–F’) Znf703 mRNA inhibits Sox10 patterning, Twist, and Tfap2a in stage 27 embryos (shown in lateral view). (D–F) Uninjected side of the same embryo. Fractions represent the portion of embryos displaying the phenotype.