Fig. 3. The cytotoxicities of compounds MI-743 and MI-929 on 16 cancer cell lines.
a Four normal cell lines and above reported cancer cell lines were treated with different concentrations of MI-743 and MI-929 for 72 h. Cell viability was determined by MTT assay, using DMSO-treated controls. IC50 values are indicated as the means ± SD of three independent experiments. TH588 and (S)-crizotinib were used as positive control. b The colony formations of MGC-803, HGC-27 and GES-1 cells were determined after being treated with 0.5, 2, 4, 8 and 10 µM of compound MI-743. At least three independent experiments were performed for each group. c, d Clonogenic survival rates of gastric cancer cell lines after MI-743 and MI-929 treatments were determined by measuring the absorbance values at 595 nm in relation to DMSO-treated controls. TH588 and (S)-crizotinib were used as positive control. Data are presented as means ± SD. Three individual experiments were performed for each group. *P < 0.05, **P < 0.01, ***P < 0.001 as compared with the controls